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Journal of Clinical Microbiology, August 2007, p. 2681-2683, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00526-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Rapid Detection Directly from Patient Serum Samples of Human Cytomegalovirus UL97 Mutations Conferring Ganciclovir Resistance{triangledown}

Jared Castor,1 Linda Cook,1 Lawrence Corey,1,2 and Keith R. Jerome1,2*

Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195,1 Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, Washington 981092

Received 8 March 2007/ Returned for modification 14 April 2007/ Accepted 20 May 2007

Ganciclovir-resistant cytomegalovirus can cause disease and death in transplant recipients. We describe here a rapid PCR- and sequencing-based assay for ganciclovir resistance that can be performed in 1 to 2 working days directly from patient specimens, without the need for amplification of the virus by cell culture. An evaluation of 120 sequential samples submitted for clinical testing revealed a variety of silent and amino acid mutations.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N, D3-100, Seattle, WA 98109. Phone: (206) 667-6793. Fax: (206) 667-4411. E-mail: kjerome{at}fhcrc.org

{triangledown} Published ahead of print on 30 May 2007.

Journal of Clinical Microbiology, August 2007, p. 2681-2683, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00526-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2007 by the American Society for Microbiology. All rights reserved.