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Journal of Clinical Microbiology, August 2007, p. 2698-2700, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00759-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Quality Control of Direct Molecular Diagnostics for Methicillin-Resistant Staphylococcus aureus{triangledown}

Alex van Belkum,1* Hubert G. M. Niesters,2,{dagger} William G. MacKay,3 and Willem B. van Leeuwen1

Department of Medical Microbiology and Infectious Diseases, Unit Research and Development,1 Department of Virology, Erasmus MC, 's-Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands,2 the Neutral Office, Quality Control for Molecular Diagnostics, Block 4, Kelvin Campus, West of Scotland Science Park, Glasgow G20 OSP, Scotland3

Received 10 April 2007/ Returned for modification 12 May 2007/ Accepted 11 June 2007

Ten samples containing various amounts of methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus, methicillin-resistant Staphylococcus epidermidis (MRSE), and combinations thereof were distributed to 51 laboratories for molecular diagnostics testing. Samples containing 102 to 103 MRSA cells were frequently reported to be negative. MRSE samples were scored as negative by all commercial tests but by only two out of three in-house tests.

* Corresponding author. Mailing address: Department of Medical Microbiology and Infectious Diseases, Unit Research and Development, Erasmus MC, 's-Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands. Phone: 00-31-10-4635813. Fax: 00-31-10-4633875. E-mail: a.vanbelkum{at}erasmusmc.nl

{triangledown} Published ahead of print on 20 June 2007.

{dagger} Present address: Department of Medical Microbiology, Section Clinical Virology Hanzeplein 1, University Medical Center Groningen, P.O. Box 30 001, 9700 RB Groningen, The Netherlands.

Journal of Clinical Microbiology, August 2007, p. 2698-2700, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00759-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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