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Journal of Clinical Microbiology, August 2007, p. 2723-2725, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00015-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of Methods To Identify the Klebsiella pneumoniae Carbapenemase in Enterobacteriaceae{triangledown}

K. F. Anderson,* D. R. Lonsway, J. K. Rasheed, J. Biddle, B. Jensen, L. K. McDougal, R. B. Carey, A. Thompson, S. Stocker, B. Limbago, and J. B. Patel

Centers for Disease Control and Prevention, Division of Healthcare Quality Promotion, Atlanta, Georgia

Received 3 January 2007/ Returned for modification 13 February 2007/ Accepted 6 June 2007

The Klebsiella pneumoniae carbapenem (KPC) ß-lactamase occurs in Enterobacteriaceae and can confer resistance to all ß-lactam agents including carbapenems. The enzyme may confer low-level carbapenem resistance, and the failure of susceptibility methods to identify this resistance has been reported. Automated and nonautomated methods for carbapenem susceptibility were evaluated for identification of KPC-mediated resistance. Ertapenem was a more sensitive indicator of KPC resistance than meropenem and imipenem independently of the method used. Carbapenemase production could be confirmed with the modified Hodge test.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, Mail Stop G-08, 1600 Clifton Road NE, Atlanta, GA 30333. Phone: (404) 639-2824. Fax: (404) 639-1381. E-mail: ebi2{at}cdc.gov

{triangledown} Published ahead of print on 20 June 2007.

Journal of Clinical Microbiology, August 2007, p. 2723-2725, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00015-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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