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Journal of Clinical Microbiology, September 2007, p. 2808-2812, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00202-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of the Abbott 7000 and Bayer 340 Systems for Measurement of Hepatitis C Virus Load{triangledown}

Helene C. Highbarger,1* Zonghui Hu,2 Shyam Kottilil,2 Julia A. Metcalf,2 Michael A. Polis,2 M. B. Vasudevachari,1 H. Clifford Lane,2 and Robin L. Dewar1

Virus Isolation and Serology Laboratory, SAIC—Frederick, Inc., NCI—Frederick, Frederick, Maryland 21702,1 Laboratory of Immunoregulation, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland 208922

Received 16 January 2007/ Returned for modification 19 March 2007/ Accepted 20 June 2007

This study compared two commercially available assays for the measurement of hepatitis C virus (HCV) RNA levels, the Bayer HCV RNA (version 3.0) branched DNA assay and the Abbott HCV analyte-specific reagent real-time PCR assay, to assess their quantitative relationships, ease of performance, and time to completion. The study group consisted of randomly selected patients from the NIAID human immunodeficiency virus (HIV) outpatient clinic who were infected with HIV type 1 and HCV. One hundred eighty-four samples from 66 patients coinfected with HIV and HCV receiving treatments under various protocols were analyzed for the correlation and agreement of the results. The results indicated that the two assays correlate well in the overlapping linear ranges of the assays and show good agreement. From the results obtained, we have derived a mathematical formula to compare the viral load results between the two assays, which is given as log10 Abbott assay measure = 0.032 + 1.01 log10 Bayer assay measure. Although it is preferable to use the same quantitation assay throughout the course of a patient's treatment, valid comparisons of the HCV RNA levels may be made between the results obtained by either of these assays in the overlapping linear range (615 to 7,700,000 IU/ml).

* Corresponding author. Mailing address: Virus Isolation and Serology Laboratory, SAIC—Frederick, Inc., NCI—Frederick, Frederick, MD 21702. Phone: (301) 846-7214. Fax: (301) 846-6762. E-mail: hhighbarge{at}mail.nih.gov

{triangledown} Published ahead of print on 27 June 2007.

Journal of Clinical Microbiology, September 2007, p. 2808-2812, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00202-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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