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Changes in the Clonal Nature and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Isolates Associated with Spread of the EMRSA-15 Clone in a Tertiary Care Portuguese Hospital

M. L. Amorim,^1,2,3, N. A. Faria,^1, D. C. Oliveira,¹ C. Vasconcelos,⁴ J. C. Cabeda,³ A. C. Mendes,^2,3 E. Calado,² A. P. Castro,² M. H. Ramos,² J. M. Amorim,² and H. de Lencastre^1,5*

Laboratório de Genética Molecular, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB/UNL), Oeiras, Portugal,¹ Serviço de Microbiologia, Hospital Geral de Santo António, Porto, Portugal,² Unidade de Biologia Molecular, Hospital Geral de Santo António, Porto, Portugal,³ Comissão de Controlo de Infecção, Hospital Geral de Santo António, Porto, Portugal,⁴ Laboratory of Microbiology, The Rockefeller University, New York, New York 10021⁵

Received 19 March 2007/ Returned for modification 9 May 2007/ Accepted 1 July 2007

Two hundred eighty methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates recovered from a tertiary care hospital in Oporto, Portugal, between 2003 and 2005 were studied by a combination of molecular typing techniques in order to investigate the genetic backgrounds associated with the changes in the resistance phenotypes observed since 2001 and compare them to those previously found in the hospital. All MRSA isolates were grouped into resistance profiles for a panel of seven antibiotics and characterized by pulsed-field gel electrophoresis (PFGE) and SCCmec (staphylococcal cassette chromosome mec) typing. Representative isolates of PFGE types were further studied by spa typing and multilocus sequence typing. Our findings clearly document that the increasing isolation of nonmultiresistant MRSA strains was associated with the decline (from 69% in 1996 to 2000 to 12% in 2003 to 2005) and massive replacement of the multiresistant Brazilian clone (ST239-IIIA) by the epidemic EMRSA-15 clone (ST22-IV), in which resistance to antibiotics other than ß-lactams is very rare, as the major clone (80% of isolates). The Iberian clone (ST247-IA), a major clone in 1992 to 1993, was represented in the present study by just one isolate. Two other pandemic MRSA clones were detected, as sporadic isolates, for the first time in our hospital: the New York/Japan (ST5-II) and the EMRSA-16 (ST36-II) clones. Furthermore, the pattern of susceptibility of MRSA isolates both to gentamicin and to trimethoprim-sulfamethoxazole was shown to be an excellent phenotypic marker for the discrimination of the EMRSA-15 clone from other nonmultiresistant MRSA clones present in our hospital.

Published ahead of print on 11 July 2007.

M.L.A. and N.A.F. contributed equally to this work.

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