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Journal of Clinical Microbiology, September 2007, p. 2993-2998, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00670-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Performance of the BD GeneOhm Methicillin-Resistant Staphylococcus aureus Test before and during High-Volume Clinical Use{triangledown}

Suzanne M. Paule,1* Donna M. Hacek,1 Bridget Kufner,1 Karine Truchon,2 Richard B. Thomson Jr.,1,3 Karen L. Kaul,1,4 Ari Robicsek,3,4 and Lance R. Peterson1,3,4

Departments of Pathology and Laboratory Medicine,1 Medicine, Evanston Northwestern Healthcare, Evanston,3 Feinberg School of Medicine, Northwestern University, Chicago,4 Illinois, and BD Diagnostics, San Diego, California2

Received 26 March 2007/ Returned for modification 5 June 2007/ Accepted 2 July 2007

We evaluated the use of the BD GeneOhm MRSA real-time PCR assay (BD Diagnostics, San Diego, CA) for the detection of nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA). The initial evaluation consisted of 403 paired nasal swabs and was done using the specimen preparation provided with the kit and an in-house lysis method that was specifically developed to accommodate large-volume testing using a minimal amount of personnel time. One swab was placed in an achromopeptidase (ACP) lysis solution, and the other was first used for culture and then prepared according to the kit protocol. PCR was performed on both lysates, and results were compared to those for culture. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the PCR assay were 98%, 96%, 77%, and 99.7% with the kit lysate and 98%, 95%, 75%, and 99.7% with the ACP lysate (P, not significant), respectively. The second evaluation was done after implementation of all-admission surveillance using PCR with ACP lysis and a sampling of 1,107 PCR-negative samples and 215 PCR-positive samples that were confirmed by culture. The results of this sampling showed an NPV of 99.9% and a PPV of 73.5% (prevalence, 6%), consistent with our initial findings. The BD GeneOhm MRSA assay is an accurate and rapid way to detect MRSA nasal colonization. When one is dealing with large specimen numbers, the ACP lysis method offers easier processing without negatively affecting the sensitivity or specificity of the PCR assay.

* Corresponding author. Mailing address: Evanston Northwestern Healthcare, Department of Pathology and Laboratory Medicine, 2650 Ridge Avenue, Evanston, IL 60201. Phone: (847) 570-2034. Fax: (847) 733-5314. E-mail: spaule{at}enh.org

{triangledown} Published ahead of print on 11 July 2007.

Journal of Clinical Microbiology, September 2007, p. 2993-2998, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00670-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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