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Journal of Clinical Microbiology, September 2007, p. 3058-3061, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00715-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Stability of Multiple-Locus Variable-Number Tandem Repeats in Salmonella enterica Serovar Typhimurium{triangledown}

K. L. Hopkins,1* C. Maguire,1 E. Best,1 E. Liebana,2 and E. J. Threlfall1

Laboratory of Enteric Pathogens, Health Protection Agency Centre for Infections, London, United Kingdom,1 Veterinary Laboratories Agency, Addlestone, Surrey, United Kingdom2

Received 2 April 2007/ Returned for modification 18 June 2007/ Accepted 25 June 2007

Variable-number tandem repeats (VNTRs) may evolve so rapidly that multiple profiles emerge during an outbreak. A total of 190 isolates from eight Salmonella enterica serovar Typhimurium outbreaks and 15 isolates from seven patients were analyzed by pulsed-field gel electrophoresis and VNTR typing. Small changes in loci were noted; otherwise, the VNTR profiles were stable during the course of the outbreaks.

* Corresponding author. Mailing address: Salmonella Reference Unit, Laboratory of Enteric Pathogens, 61 Colindale Avenue, London NW9 5EQ, United Kingdom. Phone: 44 (0) 208 327 6107. Fax: 44 (0) 208 905 9929. E-mail: katie.hopkins{at}hpa.org.uk

{triangledown} Published ahead of print on 3 July 2007.

Journal of Clinical Microbiology, September 2007, p. 3058-3061, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00715-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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