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Journal of Clinical Microbiology, September 2007, p. 3068-3071, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00646-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

PCR-Based Identification of Common Colonization Factor Antigens of Enterotoxigenic Escherichia coli{triangledown}

Abhisek Ghosal, Rudra Bhowmick, Ranjan Kumar Nandy, T. Ramamurthy, and Nabendu Sekhar Chatterjee*

National Institute of Cholera and Enteric Diseases, Kolkata, India

Received 23 March 2007/ Returned for modification 21 May 2007/ Accepted 20 June 2007

Colonization factor antigens (CFAs) of enterotoxigenic Escherichia coli (ETEC) have been classified into several groups based on their distinct antigenicity. We describe here a PCR-based method to detect common CFAs of ETEC, which were characterized using conventional serology. This PCR assay is simple and sensitive for the detection of expressed CFA genes.

* Corresponding author. Mailing address: Division of Biochemistry, National Institute of Cholera and Enteric Diseases, P33 CIT Road, Scheme XM, Beliaghata, Kolkata 700 010, India. Phone: 91(33) 2363-3372. Fax: 91(33) 2350-5066. E-mail: nschatterjee{at}rediffmail.com

{triangledown} Published ahead of print on 27 June 2007.

Journal of Clinical Microbiology, September 2007, p. 3068-3071, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00646-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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