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Journal of Clinical Microbiology, September 2007, p. 3077-3081, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00111-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Impact of Hepatitis C Virus (HCV) Genotypes on Quantification of HCV RNA in Serum by COBAS AmpliPrep/COBAS TaqMan HCV Test, Abbott Antiretroviral Therapy HCV RealTime Assay, and VERSANT HCV RNA Assay{triangledown}

Edouard Tuaillon,1 Anne-Marie Mondain,1 Laure Ottomani,1 Laurent Roudière,1 Pascal Perney,2 Marie-Christine Picot,4 Fabienne Séguret,4 François Blanc,2 Dominique Larrey,3 Philippe Van de Perre,1 and Jacques Ducos1*

Unité Hépatites Virales, Laboratoire de Virologie, Hôpital Lapeyronie,1 Service de Médecine Interne E,2 Institut des Maladies de l'Appareil Digestif, Hôpital Saint-Eloi,3 Département de l'Informatique Médicale Hôpital Arnaud de Villeneuve, CHU de Montpellier, France4

Received 16 January 2007/ Returned for modification 3 May 2007/ Accepted 11 June 2007

The VERSANT HCV RNA 3.0 (bDNA), COBAS AmpliPrep/COBAS TaqMan HCV, and Abbott ART HCV RealTime assays were compared for hepatitis C virus RNA quantification in 158 clinical specimens (genotypes 1 to 5). RNA values differed significantly between methods (P < 0.0001), and mean titer differences ranged from 0.01 to 0.50 log10 IU/ml depending on the genotypes.

* Corresponding author. Mailing address: Unité Hépatites Virales, Laboratoire de Virologie, Hôpital Lapeyronie, av. du Doyen G. Giraud, 34295 Montpellier, France. Phone: 33 467 338 336. Fax: 33 467 338 334. E-mail: j-ducos{at}chu-montpellier.fr

{triangledown} Published ahead of print on 20 June 2007.

Journal of Clinical Microbiology, September 2007, p. 3077-3081, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00111-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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Copyright © 2007 by the American Society for Microbiology. All rights reserved.