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Journal of Clinical Microbiology, September 2007, p. 3095-3097, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00643-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Characterization of Tunisian Mycobacterium tuberculosis Rifampin-Resistant Clinical Isolates{triangledown}

Alya Soudani,1* Sondess Hadjfredj,2 Mériem Zribi,1 Afef Masmoudi,1 Taieb Messaoud,2 Hanéne Tiouri,3 and Chedlia Fendri1

Bacteriology Laboratory, Rabta University Hospital, Tunis,1 Biochemistry and Molecular Laboratory, Children's Hospital, Tunis,2 Infectious Diseases Unit, Rabta University Hospital, Tunis, Tunisia3

Received 22 March 2007/ Returned for modification 10 May 2007/ Accepted 16 July 2007

Analysis of the gene encoding the ß-subunit of Mycobacterium tuberculosis RNA polymerase (rpoB) has demonstrated a small region that harbors the mutations most frequently associated with rifampin resistance. In this study, we determined the occurrence of rifampin resistance in 544 Tunisian clinical M. tuberculosis strains isolated in a university hospital between 2004 and 2006 by using the standard-proportion agar method, the INNO-LiPA Rif.TB assay, and DNA sequencing.

* Corresponding author. Mailing address: 1007 El Jabbary, Rabta University Hospital, Bacteriology Laboratory, Tunis, Tunisia. Phone: 21622602107. Fax: 21679219365. E-mail: alya_soudani{at}yahoo.fr

{triangledown} Published ahead of print on 25 July 2007.

Journal of Clinical Microbiology, September 2007, p. 3095-3097, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00643-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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