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Journal of Clinical Microbiology, September 2007, p. 3105-3108, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00976-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Revised Approach for Identification of Isolates within the Burkholderia cepacia Complex and Description of Clinical Isolates Not Assigned to Any of the Known Genomovars{triangledown}

Jane F. Turton,* Nazia Arif, Daneeta Hennessy, Mary E. Kaufmann, and Tyrone L. Pitt

Laboratory of HealthCare Associated Infection, Health Protection Agency, London, United Kingdom

Received 10 May 2007/ Returned for modification 18 June 2007/ Accepted 3 July 2007

One hundred thirty-eight clinical isolates of the Burkholderia cepacia complex (Bcc) were identified using a modified strategy that involved PCR detection of the cblA gene for the ET12 lineage simultaneously with detection of the Bcc recA PCR product; recA sequence cluster analysis also was part of the strategy. Four strains could not be assigned to any of the known genomovars.

* Corresponding author. Mailing address: Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection Agency, 61 Colindale Ave., London NW9 5EQ, United Kingdom. Phone: 44 (0) 208 327 7276. Fax: 44 (0) 208 200 7449. E-mail: jane.turton{at}hpa.org.uk

{triangledown} Published ahead of print on 11 July 2007.

Journal of Clinical Microbiology, September 2007, p. 3105-3108, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00976-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lynch, K. H., Dennis, J. J. (2008). Development of a Species-Specific fur Gene-Based Method for Identification of the Burkholderia cepacia Complex. J. Clin. Microbiol. 46: 447-455 [Abstract] [Full Text]  
  • Pimentel, J. D., Dubedat, S. M., N. Dodds, E. L., Benn, R. A. V. (2007). Identification of Isolates within the Burkholderia cepacia Complex by a Multiplex recA and 16S rRNA Gene Real-Time PCR Assay. J. Clin. Microbiol. 45: 3853-3854 [Full Text]  


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