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Journal of Clinical Microbiology, September 2007, p. 3109-3110, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00697-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Testing of Diagnostic Methods for Detection of Influenza Virus for Optimal Performance in the Context of an Influenza Surveillance Network{triangledown}

Mercedes Pérez-Ruiz,1* Ruth Yeste,1 María-José Ruiz-Pérez,1 Alfonso Ruiz-Bravo,2 Manuel de la Rosa-Fraile,1 and José María Navarro-Marí1

Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Granada, Spain,1 Departamento de Microbiología, Universidad de Granada, Granada, Spain2

Received 30 March 2007/ Returned for modification 26 April 2007/ Accepted 16 July 2007

Influenza surveillance networks must detect early the viruses that will cause the forthcoming annual epidemics and isolate the strains for further characterization. We obtained the highest sensitivity (95.4%) with a diagnostic tool that combined a shell-vial assay and reverse transcription-PCR on cell culture supernatants at 48 h, and indeed, recovered the strain.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avda. Fuerzas Armadas, 2, 18014 Granada, Spain. Phone: 34 958 020010. Fax: 34 958 020169. E-mail: mercedes.perez.ruiz.sspa{at}juntadeandalucia.es

{triangledown} Published ahead of print on 25 July 2007.

Journal of Clinical Microbiology, September 2007, p. 3109-3110, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00697-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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