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Journal of Clinical Microbiology, January 2008, p. 242-248, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01468-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Prevalence, Types, and RNA Concentrations of Human Parechoviruses, Including a Sixth Parechovirus Type, in Stool Samples from Patients with Acute Enteritis{triangledown}

Sigrid Baumgarte,1,{dagger} Luciano Kleber de Souza Luna,2,{dagger} Klaus Grywna,2 Marcus Panning,2 Jan Felix Drexler,2 Claudia Karsten,3 Hans Iko Huppertz,3 and Christian Drosten2*

Laboratory of Virology, Department of Microbiological Consumer Protection, Institute of Hygiene and the Environment, Hamburg, Germany,1 Clinical Virology Group, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany,2 Prof. Hess Childrens' Hospital, Klinikum Bremen Mitte, Bremen, Germany3

Received 21 July 2007/ Returned for modification 26 September 2007/ Accepted 5 November 2007

Parechovirus epidemiology and disease association are not fully understood. Real-time reverse transcriptase PCR (RT-PCR) for all human parechoviruses (HPeV) was applied on stool samples from two groups of patients. Both groups contained patients with acute enteritis of all age groups, seen during one full year. Patients with norovirus, adenovirus, enterovirus, astrovirus, or rotavirus infections were excluded. In 118 patients from outbreak and hospital settings, no HPeV was detected. In a prospective study group of 499 nonhospitalized patients, the detection rate was 1.6%. One virus-positive patient was detected from 39 control patients. Positive samples occurred only in summer and autumn. Only one patient had accompanying respiratory symptoms. An association with travel or animal contact was not found. All positive patients except one were <2 years of age, with a neutral gender ratio. In children <2 years of age, the detection rate was 11.6% (7 of 60 children). The range of viral loads was 3,170 to 503,377,290 copies per gram or milliliter of stool. One of the highest viral loads occurred in a control child without symptoms at the time of testing. Phylogenetic analysis showed mainly contemporary HPeV1 strains in our patients but also showed a separate new lineage of HPeV1 in evolutionary transition from the historical prototype strain. Moreover, a novel sixth HPeV type was identified. Full genome analysis of the two viruses revealed recombination between HPeV1 and -3 in one and HPeV6 and -1 in another. HPeV seems relevant in children <2 years and specific RT-PCR for HPeV should be included in enteritis screening.

* Corresponding author. Present address: Institute of Virology, University of Bonn Medical Centre, Sigmund Freud-Str. 25, 53127 Bonn, Germany. Phone: 49-228-287-11055. Fax: 49-228-287-19144. E-mail: drosten{at}virology-bonn.de

{triangledown} Published ahead of print on 5 December 2007.

{dagger} These authors have contributed equally (alphabetical order).

Journal of Clinical Microbiology, January 2008, p. 242-248, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01468-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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