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Journal of Clinical Microbiology, January 2008, p. 286-289, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01172-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Nested PCR for Specific Diagnosis of Taenia solium Taeniasis{triangledown}

Holger Mayta,1,2 Robert H. Gilman,1,2* Emily Prendergast,3 Janeth P. Castillo,2 Yeny O. Tinoco,4 Hector H. Garcia,2,5 Armando E. Gonzalez,4 Charles R. Sterling,3 for the Cysticercosis Working Group in Peru

Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland,1 Department of Microbiology, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru,2 Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona,3 School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos,4 Department of Transmissible Diseases, Instituto Nacional de Ciencias Neurologicas, Lima, Peru5

Received 11 June 2007/ Returned for modification 20 August 2007/ Accepted 28 October 2007

Taeniasis due to Taenia solium is a disease with important public health consequences, since the larval stage is not exclusive to the animal intermediate, the pig, but also infects humans, causing neurocysticercosis. Early diagnosis and treatment of T. solium tapeworm carriers is important to prevent human cysticercosis. Current diagnosis based on microscopic observation of eggs lacks both sensitivity and specificity. In the present study, a nested-PCR assay targeting the Tso31 gene was developed for the specific diagnosis of taeniasis due to T. solium. Initial specificity and sensitivity testing was performed using stored known T. solium-positive and -negative samples. The assay was further analyzed under field conditions by conducting a case-control study of pretreatment stool samples collected from a population in an area of endemicity. Using the archived samples, the assay showed 97% (31/32) sensitivity and 100% (123/123) specificity. Under field conditions, the assay had 100% sensitivity and specificity using microscopy/enzyme-linked immunosorbent assay coproantigen testing as the gold standards. The Tso31 nested PCR described here might be a useful tool for the early diagnosis and prevention of taeniasis/cysticercosis.

* Corresponding author. Mailing address: Department of International Health, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street W5515, Baltimore, MD 21205. Phone: (410) 614-3959. Fax: (410) 614-6060. E-mail: Rgilman{at}jhsph.edu

{triangledown} Published ahead of print on 7 November 2007.

Journal of Clinical Microbiology, January 2008, p. 286-289, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01172-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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