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Journal of Clinical Microbiology, January 2008, p. 328-330, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01503-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rapid and Reliable Diagnostic Algorithm for Detection of Clostridium difficile{triangledown}

Lukas Fenner,1 Andreas F. Widmer,2 Gisela Goy,1 Sonja Rudin,1 and Reno Frei1*

Microbiology Laboratory,1 Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Petersgraben 4, CH-4031 Basel, Switzerland2

Received 26 July 2007/ Returned for modification 20 September 2007/ Accepted 2 November 2007

We evaluated a two-step algorithm for detection of Clostridium difficile in 1,468 stool specimens. First, specimens were screened by an immunoassay for C. difficile glutamate dehydrogenase antigen (C.DIFF CHEK-60). Second, screen-positive specimens underwent toxin testing by a rapid toxin A/B assay (TOX A/B QUIK CHEK); toxin-negative specimens were subjected to stool culture. This algorithm allowed final results for 92% of specimens with a turnaround time of 4 h.

* Corresponding author. Mailing address: Microbiology Laboratory, University Hospital Basel, Petersgraben 4, CH-4031 Basel, Switzerland. Phone: 41 61 265 42 44. Fax: 41 61 265 53 55. E-mail: rfrei{at}uhbs.ch

{triangledown} Published ahead of print on 21 November 2007.

Journal of Clinical Microbiology, January 2008, p. 328-330, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01503-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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