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Journal of Clinical Microbiology, January 2008, p. 331-333, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01251-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Analysis of Toxigenic Corynebacterium ulcerans Strains Revealing Potential for False-Negative Real-Time PCR Results{triangledown}

Pamela K. Cassiday,1* Lucia C. Pawloski,1 Tejpratap Tiwari,2 Gary N. Sanden,1 and Patricia P. Wilkins1

National Center for Infectious Diseases,1 National Immunization Program, Centers for Disease Control and Prevention, Atlanta, Georgia2

Received 21 June 2007/ Returned for modification 31 August 2007/ Accepted 28 October 2007

Diphtheria surveillance depends on the rapid and reliable recognition of the toxin gene in Corynebacterium diphtheriae. Real-time PCR is a rapid tool to confirm the presence of the diphtheria toxin gene (tox) in an isolate or specimen. We report that some toxigenic Corynebacterium ulcerans strains show atypical results in a real-time PCR for tox.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop D-11, Atlanta, GA 30333. Phone: (404) 639-1231. Fax: (404) 639-4421. E-mail: PCassiday{at}cdc.gov

{triangledown} Published ahead of print on 7 November 2007.

Journal of Clinical Microbiology, January 2008, p. 331-333, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01251-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schuhegger, R., Lindermayer, M., Kugler, R., Heesemann, J., Busch, U., Sing, A. (2008). Detection of Toxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans Strains by a Novel Real-Time PCR. J. Clin. Microbiol. 46: 2822-2823 [Full Text]  


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