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Journal of Clinical Microbiology, January 2008, p. 346-349, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01670-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Hidden VIM-1 Metallo-β-Lactamase Phenotypes among Acinetobacter baumannii Clinical Isolates{triangledown}

Alexandros Ikonomidis,1 Eleni Ntokou,1 Antonios N. Maniatis,1 Athanassios Tsakris,2 and Spyros Pournaras1*

Department of Microbiology, Medical School, University of Thessaly, Larissa,1 Department of Microbiology, Medical School, University of Athens, Athens, Greece2

Received 22 August 2007/ Returned for modification 15 October 2007/ Accepted 5 November 2007

A total of 87 Acinetobacter baumannii nonrepetitive consecutive clinical isolates were tested for the presence of metallo-β-lactamases (MBLs). Results of phenotypic assays (MBL Etest, imipenem/imipenem-EDTA combined-disk test, and imipenem/EDTA double-disk synergy test) were negative in all cases, but molecular testing revealed the presence of two blaVIM-1-carrying isolates. One isolate had blaVIM-1 preceded by a weak P1 promoter, and both had inactivated P2 promoters and reduced blaVIM-1 expression, partially justifying the results revealing hidden MBL phenotypes.

* Corresponding author. Mailing address: Department of Medical Microbiology, University Hospital of Larissa, Mezourlo, 411 10 Larissa, Greece. Phone: 30 2410 682537. Fax: 30 2410 681570. E-mail: pournaras{at}med.uth.gr

{triangledown} Published ahead of print on 21 November 2007.

Journal of Clinical Microbiology, January 2008, p. 346-349, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01670-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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  • Loli, A., Tzouvelekis, L. S., Gianneli, D., Tzelepi, E., Miriagou, V. (2008). Outbreak of Acinetobacter baumannii with Chromosomally Encoded VIM-1 Undetectable by Imipenem-EDTA Synergy Tests. Antimicrob. Agents Chemother. 52: 1894-1896 [Full Text]  


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