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Journal of Clinical Microbiology, January 2008, p. 366-368, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01323-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Improvement of PCR for Detection of Opisthorchis viverrini DNA in Human Stool Samples{triangledown}

Kunyarat Duenngai,1,2 Paiboon Sithithaworn,1,2* Umesha Kanasinakatte Rudrappa,3 Karunasagar Iddya,3 Thewarach Laha,1 Christen Rune Stensvold,4 Hanne Strandgaard,4 and Maria Vang Johansen4

Department of Parasitology,1 Liver Fluke and Cholangiocarcinoma Research Center (LFCRC), Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand,2 Department of Fishery Microbiology, University of Agricultural Sciences, College of Fisheries, Mangalore 575 002, India,3 DBL—Center for Health Research and Development, DK-2920 Charlottenlund, Denmark4

Received 2 July 2007/ Returned for modification 10 September 2007/ Accepted 31 October 2007

Opisthorchis viverrini is an important food-borne trematode in Southeast Asia. The infection causes significant morbidity in terms of hepatobiliary diseases and cholangiocarcinoma. The aim of this study was to improve the sensitivity of the PCR-based diagnosis of O. viverrini infection. A new fecal DNA extraction protocol for the detection of O. viverrini DNA using cetyltrimethyl-ammoniumbromide to remove PCR inhibitor was used and compared with the commercial stool kit method. The sensitivity of the new test was 79.3%, compared with the 44.8% of the previous method (P < 0.01). PCR-positive tests identified several cases judged parasite negative by the parasitological method (28.6%), indicating the new test's advantage in the diagnosis of individuals with light infections.

* Corresponding author. Mailing address: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. Phone: 66-43-348387. Fax: 66-43-202475. E-mail: paib_sit{at}kku.ac.th

{triangledown} Published ahead of print on 14 November 2007.

Journal of Clinical Microbiology, January 2008, p. 366-368, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01323-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lovis, L., Mak, T. K., Phongluxa, K., Soukhathammavong, P., Sayasone, S., Akkhavong, K., Odermatt, P., Keiser, J., Felger, I. (2009). PCR Diagnosis of Opisthorchis viverrini and Haplorchis taichui Infections in a Lao Community in an Area of Endemicity and Comparison of Diagnostic Methods for Parasitological Field Surveys. J. Clin. Microbiol. 47: 1517-1523 [Abstract] [Full Text]  


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