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Journal of Clinical Microbiology, January 2008, p. 385-387, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01303-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

CASE REPORT

Rapid Diagnosis of Human Brucellosis by Quantitative Real-Time PCR: a Case Report of Brucellar Spondylitis{triangledown}

Amalia Navarro-Martínez,1 Elena Navarro,2 Maria Jesús Castaño,2 and Javier Solera1*

Internal Medicine Department, University General Hospital, c/ Hermanos Falcó s/n, 02006, Albacete, Spain,1 Experimental Research Unit, University General Hospital, c/ Hermanos Falcó s/n, 02006, Albacete, Spain2

Received 28 June 2007/ Returned for modification 20 August 2007/ Accepted 15 October 2007

Blood samples and a bone biopsy specimen from one patient diagnosed with spondylodiscitis of unknown etiology were analyzed by quantitative real-time PCR to detect Brucella melitensis. The high sensitivity and specificity of this assay allowed the diagnosis of brucellar spondylitis within 24 h, a result that we were unable to obtain through the use of conventional methods.

* Corresponding author. Mailing address: Servicio de Medicina Interna, Hospital General Universitario, c/ Hermanos Falcó s/n., 02006 Albacete, Spain. Phone: 34-67-597215. Fax: 34-67-243952. E-mail: solera53{at}yahoo.es

{triangledown} Published ahead of print on 31 October 2007.

Journal of Clinical Microbiology, January 2008, p. 385-387, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01303-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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