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Journal of Clinical Microbiology, January 2008, p. 43-49, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01494-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Novel Brucella Strain (BO1) Associated with a Prosthetic Breast Implant Infection{triangledown}

Barun K. De,1* Larry Stauffer,2 Mark S. Koylass,3 Susan E. Sharp,4 Jay E. Gee,1 Leta O. Helsel,1 Arnold G. Steigerwalt,1 Robert Vega,2 Thomas A. Clark,1 Maryam I. Daneshvar,1 Patricia P. Wilkins,1 and Adrian M. Whatmore3

Centers for Disease Control and Prevention, Atlanta, Georgia 30333,1 Oregon State Public Health Lab, Portland, Oregon 97201,2 Veterinary Laboratories Agency, Addlestone, Surrey, United Kingdom,3 Kaiser Permanente, Portland, Oregon 972304

Received 25 July 2007/ Returned for modification 9 September 2007/ Accepted 21 October 2007

We report the microbiological, biochemical, and molecular characterization of an unusual Brucella strain (BO1) isolated from a breast implant wound in a 71-year-old woman with clinical symptoms consistent with brucellosis. Initial phenotypic analysis, including biochemical and antimicrobial susceptibility testing, cellular fatty acid analysis, and molecular analysis based on DNA-DNA reassociation and the presence of multiple copies of IS711 element suggested that the isolate was a Brucella-like organism, but species determination using microbiological algorithms was unsuccessful. Furthermore, molecular data based on 16S rRNA gene sequencing and multilocus sequence analysis demonstrated that BO1 was an unusual Brucella strain and not closely related to any currently described Brucella species. However, comparison with equivalent sequences in Ochrobactrum spp. confirms that the isolate is much more closely related to Brucella than to Ochrobactrum spp., and thus the isolate likely represents an atypical and novel strain within the genus Brucella.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, Mail Stop G34, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-5465. Fax: (404) 639-3023. E-mail: bkd1{at}cdc.gov

{triangledown} Published ahead of print on 31 October 2007.

Journal of Clinical Microbiology, January 2008, p. 43-49, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01494-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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