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Journal of Clinical Microbiology, October 2008, p. 3470-3472, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.00858-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Detection of Group B Streptococci in Lim Broth by Use of Group B Streptococcus Peptide Nucleic Acid Fluorescent In Situ Hybridization and Selective and Nonselective Agars{triangledown}

Naomi S. Montague,1 Timothy J. Cleary,1 Octavio V. Martinez,2 and Gary W. Procop3*

Department of Pathology, Jackson Memorial Hospital, and the University of Miami Miller School of Medicine, Miami, Florida,1 Department of Orthopedics, University of Miami Miller School of Medicine, Miami, Florida,2 Section of Clinical Microbiology, Department of Clinical Pathology, Cleveland Clinic, Cleveland, Ohio3

Received 5 May 2008/ Returned for modification 29 May 2008/ Accepted 21 July 2008

The sensitivity, specificity, and positive and negative predictive values for the detection of group B streptococci from Lim enrichment broth with sheep blood agar (SBA), with selective Streptococcus agar (SSA), and by a peptide nucleic acid fluorescent in situ hybridization (PNA FISH) assay were as follows: for culture on SBA, 68.4%, 100%, 100%, and 87.9%, respectively; for culture on SSA, 85.5%, 100%, 100%, and 94.1%, respectively; and for the PNA FISH assay, 97.4%, 98.3%, 96.1%, and 98.9%, respectively.

* Corresponding author. Mailing address: Clinical Pathology, Cleveland Clinic, 9500 Euclid Avenue/L11, Cleveland, OH 44195. Phone: (216) 444-5879. Fax: (216) 444-4414. E-mail: procopg{at}ccf.org

{triangledown} Published ahead of print on 30 July 2008.

Journal of Clinical Microbiology, October 2008, p. 3470-3472, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.00858-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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