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Journal of Clinical Microbiology, October 2008, p. 3526-3529, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.02083-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Inter- and Intra-Assay Reproducibility of Microplate Alamar Blue Assay Results for Isoniazid, Rifampicin, Ethambutol, Streptomycin, Ciprofloxacin, and Capreomycin Drug Susceptibility Testing of Mycobacterium tuberculosis{triangledown} ,{dagger}

Brian Leonard,1 Jorge Coronel,1 Mark Siedner,1 Louis Grandjean,1 Luz Caviedes,1 Pilar Navarro,1 Robert H. Gilman,1,2,3,4 and David A. J. Moore1,2,3,4*

Laboratorio de Investigación de Enfermedades Infecciosas, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado No. 430, San Martín de Porras, Lima 31, Perú,1 Department of International Health, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe St., Room 5515, Baltimore, Maryland 21205,2 Asociación Benéfica PRISMA, Carlos Gonzales No. 251, San Miguel, Lima 32, Perú,3 Department of Infectious Diseases and Immunity and Wellcome Centre for Clinical Tropical Medicine, Faculty of Medicine (Hammersmith Campus), Imperial College London, DuCane Road, London W12 0NN, United Kingdom4

Received 27 October 2007/ Returned for modification 4 February 2008/ Accepted 8 July 2008

The intersample and intrasample variability of the results obtained with the microplate Alamar blue assay for the indirect drug susceptibility testing of Mycobacterium tuberculosis was investigated. Between 1.2 and 8.5% of paired MICs differed by more than one twofold dilution, resulting in discordant susceptible-resistant designations at frequencies between 0.6% (rifampin) and 18.9% (ethambutol).

* Corresponding author. Mailing address: Laboratorio de Investigación de Enfermedades Infecciosas, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado No. 430, San Martín de Porras, Lima 31, Perú. Phone: 511 382 3398. Fax: 511 464 0781. E-mail: dajmoore{at}imperial.ac.uk

{triangledown} Published ahead of print on 13 August 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, October 2008, p. 3526-3529, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.02083-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Carroll, J., Douarre, P., Coffey, A., Buckley, J., Cashman, B., O'Farrell, K., O'Mahony, J. (2009). Optimization of a Rapid Viability Assay for Mycobacterium avium subsp. paratuberculosis by Using alamarBlue. Appl. Environ. Microbiol. 75: 7870-7872 [Abstract] [Full Text]  


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