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Journal of Clinical Microbiology, November 2008, p. 3569-3575, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01095-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Phenotypic and Genotypic Characterization of Verotoxin-Producing Escherichia coli O103:H2 Isolates from Cattle and Humans{triangledown}

Musafiri Karama,1 Roger P. Johnson,2 Robert Holtslander,2 and Carlton L. Gyles1*

Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1,1 Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada2

Received 9 June 2008/ Returned for modification 2 August 2008/ Accepted 21 August 2008

Characterization of important non-O157 verotoxin-producing Escherichia coli (VTEC) has lagged considerably behind that of O157:H7 strains. This study characterized 91 VTEC O103:H2 strains from bovine and human sources and of North American and European origins by virulence or putative virulence genes, pulsed-field gel electrophoresis (PFGE) patterns, plasmid profiles, antimicrobial resistance, and colicin production. All strains were positive for vt1 and eae-{varepsilon}; 97% were positive for ehxA; and all were negative for hlyA. Two strains carried vt2. There were 66 PFGE patterns grouped in six clusters, and there were 25 different plasmid profiles. Plasmid-encoded katP and etp genes were significantly more frequent in European than in North American human strains. The distribution of selected phenotypes was as follows: enterohemorrhagic E. coli (EHEC) hemolysin, 95%; colicin production, 38%; antimicrobial resistance, 58%. All the strains were negative for the alpha-hemolytic phenotype. In conclusion, the VTEC O103:H2 strains were diverse, as shown by PFGE, plasmid profiles, virulence markers, and antimicrobial resistance patterns, and all strains showed an EHEC hemolytic phenotype instead of the alpha-hemolytic phenotype that has been shown previously.

* Corresponding author. Mailing address: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2W1, Canada. Phone: (519) 824-4120, ext. 54657. Fax: (519) 824-5930. E-mail: cgyles{at}uoguelph.ca

{triangledown} Published ahead of print on 3 September 2008.

Journal of Clinical Microbiology, November 2008, p. 3569-3575, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01095-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Karama, M., Johnson, R. P., Holtslander, R., Gyles, C. L. (2009). Production of Verotoxin and Distribution of O Islands 122 and 43/48 among Verotoxin-Producing Escherichia coli O103:H2 Isolates from Cattle and Humans. Appl. Environ. Microbiol. 75: 268-270 [Abstract] [Full Text]  


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