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Journal of Clinical Microbiology, November 2008, p. 3595-3598, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01635-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

DNA from BK Virus and JC Virus and from KI, WU, and MC Polyomaviruses as Well as from Simian Virus 40 Is Not Detected in Non-UV-Light-Associated Primary Malignant Melanomas of Mucous Membranes {triangledown}

Géraldine Giraud,1* Torbjörn Ramqvist,1 Boel Ragnarsson-Olding,1,2 and Tina Dalianis1,3

Department of Oncology-Pathology, Karolinska Institutet, Cancer Center Karolinska,1 Department of Oncology, Radiumhemmet, Karolinska University Hospital, SE-171 76 Stockholm,2 Department of Virology, Swedish Institute for Infectious Disease Control, 171 82 Solna, Sweden3

Received 22 August 2008/ Accepted 22 August 2008

The single most important causative factor for malignant melanomas of the skin is UV radiation. However, this is not true for melanomas on body surfaces sheltered from the sun; thus, it is important to seek new causative factors of melanoma genesis. Human papillomaviruses and gammaherpesviruses are associated with human skin cancer; for example, human papillomavirus types 5 and 8 are associated with epidermodysplasia verruciformis, and human herpesvirus 8 is associated with Kaposi's sarcoma. Recently, a newly described human polyomavirus, Merkel cell polyomavirus (MCPyV), has been associated with Merkel cell carcinoma, an unusual form of neurotropic skin cancer. Moreover, melanocytes are of neuroepithelial origin. This background impelled us to investigate if human polyomavirus DNA could play a role in the development of extracutaneous melanomas. Sixty-four extracutaneous melanomas were initially collected and dissected. Of these, 38 could be successfully used for further testing for the presence of the five human polyomaviruses known so far—BK virus (BKV), JC virus (JCV), KI polyomavirus (KIPyV), WU polyomavirus (WUPyV), and MCPyV—and of simian virus 40 (SV40). No polyomavirus DNA could be detected in any of the samples tested by use of a nested PCR detecting BKV, JCV, and SV40; a newly designed PCR detecting KIPyV and WUPyV; or a newly designed PCR for MCPyV. We conclude that since no human polyomavirus DNA was detected in primary malignant melanomas on non-sun-exposed body surfaces, these polyomaviruses presumably are not major factors for the development of extracutaneous melanomas.

* Corresponding author. Mailing address: Department of Oncology-Pathology, Karolinska Institutet, Karolinska University Hospital CCK R8:01, SE-171 76 Stockholm, Sweden. Phone: 46 8 517 727 63. Fax: 46 8 517 766 30. E-mail: geraldine.giraud{at}ki.se

{triangledown} Published ahead of print on 3 September 2008.

Journal of Clinical Microbiology, November 2008, p. 3595-3598, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01635-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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