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Journal of Clinical Microbiology, November 2008, p. 3798-3799, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01551-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Use of Bordetella pertussis BP3385 To Establish a Cutoff Value for an IS481-Targeted Real-Time PCR Assay {triangledown}

J. L. Guthrie,1* C. Seah,1 S. Brown,1 P. Tang,1 F. Jamieson,1,2 and S. J. Drews1,2,3

Ontario Public Health Laboratories, Toronto, Ontario, Canada,1 Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada,2 Mt. Sinai Hospital, Toronto, Ontario, Canada3

Received 11 August 2008/ Returned for modification 2 September 2008/ Accepted 4 September 2008

This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (CT) values of >35. IS481 PCRs with CT values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.

* Corresponding author. Mailing address: Ministry of Health and Long-Term Care, 81 Resources Rd., Toronto, Ontario M9P 3T1, Canada. Phone: (416) 235-6521. Fax: (416) 235-6281. E-mail: jennifer.guthrie{at}ontario.ca

{triangledown} Published ahead of print on 10 September 2008.

Journal of Clinical Microbiology, November 2008, p. 3798-3799, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01551-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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