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Journal of Clinical Microbiology, November 2008, p. 3813-3816, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01169-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Validation of an Automated Detection Platform for Use with the Roche Linear Array Human Papillomavirus Genotyping Test{triangledown}

Matthew P. Stevens,1* Suzanne M. Garland,1,2 and Sepehr N. Tabrizi1,2

Department of Microbiology, The Royal Women's Hospital, Carlton, Victoria, Australia,1 Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, Victoria, Australia2

Received 20 June 2008/ Returned for modification 9 August 2008/ Accepted 31 August 2008

An automated platform (BeeBlot) was evaluated in parallel with the recommended protocol for the hybridization and detection steps of the Roche Linear Array human papillomavirus (HPV) genotyping test using DNA from 143 cervical specimens. Genotyping profiles showed 100% concordance between the methods, suggesting that automation could complement the Roche Linear Array for enhanced speed and reproducibility.

* Corresponding author. Mailing address: Department of Microbiology, The Royal Women's Hospital, 132 Grattan Street, Carlton, Victoria 3053, Australia. Phone: (61-3) 9344 3108. Fax: (61-3) 9344 2713. E-mail: matthew.stevens{at}mcri.edu.au

{triangledown} Published ahead of print on 10 September 2008.

Journal of Clinical Microbiology, November 2008, p. 3813-3816, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01169-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tabrizi, S. N., Stevens, M. P., van Leeuwen, M. T., Vajdic, C. M., Botes, L. P., Medley, G., Hillman, R., Grulich, A., Garland, S. M. (2009). Effect of ThinPrep Preparation on Human papillomavirus Detection and Genotyping in Rectal Samples by PCR. J. Clin. Microbiol. 47: 227-229 [Abstract] [Full Text]  


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