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Journal of Clinical Microbiology, November 2008, p. 3822-3825, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00451-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Sequencing of hsp65 Gene for Identification of Mycobacterium Species Isolated from Environmental and Clinical Sources in Rio de Janeiro, Brazil{triangledown} ,{dagger}

Simone G. Senna,1* Jaqueline Battilana,2 Juliana C. Costa,2 Marlei G. Silva,1 Rafael S. Duarte,1 Leila S. Fonseca,1 Philip N. Suffys,3 and Mauricio R. Bogo2*

Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Instituto de Microbiologia Professor Paulo de Góes, Rio de Janeiro, Brazil,1 Pontifícia Universidade Católica do Rio Grande do Sul, Faculdade de Biociências, Laboratório de Biologia Genômica e Molecular Porto Alegre, Brazil,2 Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada a Micobactérias, Rio de Janeiro, Brazil3

Received 6 March 2008/ Returned for modification 21 March 2008/ Accepted 19 August 2008

This study evaluated the biodiversity of 28 clinical and 24 environmental Mycobacterium isolates from Rio de Janeiro, Brazil, by using hsp65 sequences, with the aim of contributing to a better understanding of the genetic diversity and usefulness of this marker. An extensive phylogenetic analysis was performed. The nucleotide diversity was similar between clinical (0.06508) and environmental (0.06221) isolates.

* Corresponding author. Mailing address for Simone G. Senna: Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Instituto de Microbiologia Professor Paulo de Góes, Rio de Janeiro, Brazil. Phone: 55 21 22604193. Fax: 55 21 25608344. E-mail: simonesenna{at}hotmail.com. Mailing address for Mauricio R. Bogo: Pontifícia Universidade Católica do Rio Grande do Sul, Faculdade de Biociências, Laboratório de Biologia Genômica e Molecular, Porto Alegre, Brazil. Phone: 55 51 33203500. Fax: 55 51 33203568. E-mail: mbogo{at}pucrs.br

{triangledown} Published ahead of print on 3 September 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, November 2008, p. 3822-3825, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00451-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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