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Journal of Clinical Microbiology, November 2008, p. 3826-3828, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00877-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of Coagulase-Negative Staphylococci by Using the BD Phoenix System in the Low-Inoculum Mode{triangledown}

Gioconda Brigante,2 Maria Grazia Menozzi,3 Beatrice Pini,2 Rosaria Porta,1 Pietro Somenzi,3 Agata Sciacca,4 Teresa Spanu,1 and Stefania Stefani5*

Institute of Microbiology, Università Cattolica del Sacro Cuore, Rome,1 Laboratory of Medical Microbiology, University of Insubria and Ospedale di Circolo, Varese,2 Department of Pathology and Laboratory Medicine, Section of Microbiology, University of Parma, Parma,3 Laboratory of Microbiology, Azienda Policlinico, University of Catania, Catania,4 Department of Microbiological and Gynecological Sciences, Section of Microbiology, University of Catania, Catania, Italy5

Received 7 May 2008/ Returned for modification 27 July 2008/ Accepted 2 September 2008

The new "low-inoculum" mode of the Phoenix system was evaluated to identify clinical coagulase-negative staphylococci. API ID32 Staph panels were used as comparators, and discrepancies were resolved by 16S rRNA and tuf gene analysis. The system correctly identified 90.5% of isolates, with a mean time of 10.2 h. Accuracy was satisfactory for Staphylococcus epidermidis, S. saprophyticus, and S. haemolyticus.

* Corresponding author. Mailing address: Department of Microbiology, University of Catania, Via Androne 81, 95124 Catania, Italy. Phone: 39 095-2504714. Fax: 39 095-2504733. E-mail: stefanis{at}unict.it

{triangledown} Published ahead of print on 10 September 2008.

Journal of Clinical Microbiology, November 2008, p. 3826-3828, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00877-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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