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Journal of Clinical Microbiology, November 2008, p. 3836-3838, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01276-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rapid Differentiation of Methicillin-Resistant Staphylococcus aureus and Methicillin-Susceptible Staphylococcus aureus from Blood Cultures by Use of a Direct Cefoxitin Disk Diffusion Test {triangledown}

Kelly Bennett and Susan E. Sharp*

Kaiser Permanente, Airport Way Laboratory, 13705 N.E. Airport Way, Portland, Oregon 97230

Received 7 July 2008/ Returned for modification 18 August 2008/ Accepted 19 September 2008

A total of 276 blood culture bottles with Staphylococcus aureus were tested by direct cefoxitin disk diffusion testing; 105 (38.1%) had zone sizes of ≤17 mm (all 105 had methicillin-resistant S. aureus [MRSA]), 18 (6.5%) had zone sizes that measured 18 mm (17 had MRSA and 1 had methicillin-susceptible S. aureus [MSSA]), 8 (2.9%) had zone sizes that measured 19 mm (6 had MRSA and 2 had MSSA), 8 (2.9%) had zone sizes that measured 20 mm (6 had MRSA and 2 had MSSA), and 137 (49.6%) had zone sizes of ≥21 mm (all 137 had MSSA). Detection of MRSA/MSSA in blood cultures could be reported 10 to 24 h earlier for 88% of cultures with total accuracy.

* Corresponding author. Mailing address: Kaiser Permanente NW, 13705 N.E. Airport Way, Portland, OR 97230. Phone: (503) 258-6824. Fax: (503) 258-6864. E-mail: susan.e.sharp{at}kp.org

{triangledown} Published ahead of print on 1 October 2008.

Journal of Clinical Microbiology, November 2008, p. 3836-3838, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01276-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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