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Journal of Clinical Microbiology, December 2008, p. 3875-3879, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.00810-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of Staphylococcus spp. by PCR-Restriction Fragment Length Polymorphism Analysis of dnaJ Gene{triangledown}

Tomasz Hauschild1* and Srdjan Stepanovic2

Department of Microbiology, Institute of Biology, University of Bialystok, Bialystok 15-950, Poland,1 Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Belgrade 11000, Serbia2

Received 29 April 2008/ Returned for modification 2 May 2008/ Accepted 23 September 2008

A PCR-restriction fragment length polymorphism (RFLP) analysis method that analyzes a part of the dnaJ gene was designed for the rapid and accurate identification of Staphylococcus spp. XapI or Bsp143I digestion of the PCR-generated products rendered distinctive RFLP patterns that allowed 41 reference species and subspecies to be identified with a high degree of specificity. The novel method was validated by the identification of 23 clinical staphylococcal strains, and the results were compared with those obtained by other genotypic identification methods. A 100% concordance of the results was shown. Therefore, PCR-RFLP analysis of the dnaJ gene is proposed as a reliable and reproducible method for the identification of Staphylococcus spp.

* Corresponding author. Mailing address: Department of Microbiology, Institute of Biology, University of Bialystok, Swierkowa 20 B, Bialystok 15-950, Poland. Phone: 48 85 74 57 428. Fax: 48 85 74 57 302. E-mail: thausch{at}uwb.edu.pl

{triangledown} Published ahead of print on 1 October 2008.

Journal of Clinical Microbiology, December 2008, p. 3875-3879, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.00810-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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