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Journal of Clinical Microbiology, December 2008, p. 3900-3905, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.00734-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Absence of CTX-M Enzymes but High Prevalence of Clones, Including Clone ST131, among Fecal Escherichia coli Isolates from Healthy Subjects Living in the Area of Paris, France{triangledown}

Véronique Leflon-Guibout,1 Jorge Blanco,2 Karim Amaqdouf,1 Azucena Mora,2 Louis Guize,3 and Marie-Hélène Nicolas-Chanoine1,4*

Microbiology Department, Beaujon AP-HP Hospital, Clichy,1 Medical Center IPC,3 Inserm U773, Faculté de Médecine D. Diderot, Paris, France,4 E. coli Reference Laboratory, Department of Microbiology and Parasitology, Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, Spain2

Received 16 April 2008/ Returned for modification 30 June 2008/ Accepted 30 September 2008

Quinolone-resistant and CTX-M-15-producing Escherichia coli isolates belonging to clone ST131 have been reported in the community. This study was designed to identify these E. coli isolates in the stools of 332 independent healthy subjects living in the area of Paris, France. Stools were plated on media without antibiotics, in order to obtain the dominant (Dm) fecal E. coli strain, and with nalidixic acid (NAL) and cefotaxime. Quinolone susceptibility, phylogenetic groups, and molecular profiles, including multilocus sequence types (ST), were determined for all NAL-resistant (NAL-R) isolates. Groups were also determined for the Dm strains from participants with NAL-R isolates and from a subgroup without NAL-R isolates. All B2 isolates were typed; pulsed-field gel electrophoresis was performed for the ST131 isolates, and the results were compared with those for intercontinental clone ST131. Two participants (0.6%) had extended-spectrum β-lactamase-producing (SHV-2, TEM-52) fecal E. coli isolates, and 51 (15%) had NAL-R isolates; 51% of NAL-R isolates belonged to phylogenetic group A, 31% to group D, 16% to group B2, and 2% to group B1. The Dm strain was NAL-R in 3.3% of the 332 subjects. Forty-nine percent of the NAL-R isolates belonged to clones: ST10 and ST606 for group A isolates, ST117 and ST393 for group D isolates. Of all B2 isolates studied from 100 subjects (8 NAL-R strains; 19 NAL-susceptible dominant strains), 52% belonged to three clones: ST131 (n = 7), ST95 (n = 4), and ST141 (n = 3). This is the first study to show the presence of fecal E. coli isolates of clone ST131 in 7% of independent healthy subjects not colonized by CTX-M-15-producing isolates.

* Corresponding author. Mailing address: Hôpital Beaujon, Service de Microbiologie, 92110 Clichy, France. Phone: 33 1 40 87 56 06. Fax: 33 1 40 87 05 50. E-mail: mhn.chanoine{at}bjn.aphp.fr

{triangledown} Published ahead of print on 8 October 2008.

Journal of Clinical Microbiology, December 2008, p. 3900-3905, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.00734-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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