This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wattiau, P.
Right arrow Articles by Imberechts, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wattiau, P.
Right arrow Articles by Imberechts, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2008, p. 4037-4040, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01405-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparison of Classical Serotyping and PremiTest Assay for Routine Identification of Common Salmonella enterica Serovars{triangledown} ,{dagger}

Pierre Wattiau,* Mieke Van Hessche, Christine Schlicker, Heidi Vander Veken, and Hein Imberechts

Veterinary and Agrochemical Research Centre, Department of Bacteriology and Immunology, Groeselenberg 99, Brussels B-1180, Belgium

Received 22 July 2008/ Returned for modification 1 September 2008/ Accepted 27 September 2008

The commercial PremiTest Salmonella kit uses a multiplexed DNA typing test aimed at identifying common serovars of Salmonella enterica. It was used in assays over a 9-month period in the Belgian reference laboratory that performs the routine identification of Salmonella strains of animal origin. A blind analysis of 754 strains was conducted in parallel by classical serotyping and the PremiTest assay. Full results were available for 685 strains (90.8%) by serotyping, while the remaining 69 strains were found to be nontypeable due to either a lack of surface antigen expression or autoagglutination properties. When the PremiTest assay (version 4.2) was performed with crude bacterial extracts, it identified 658 strains (87.3%), including most strains found to be nontypeable by serotyping. In contrast, it gave no, wrong, dual, or noninterpretable results for 96 strains, for which 23 were caused by assay failures. When purified DNA instead of crude extracts were tested, the number of strains successfully identified to the serovar level increased to 714 (94.7%), while all assay failures were cleared. Our conclusion is that, in its actual development stage, the application of the investigated kit to purified DNA samples offers a valuable alternative to classical serotyping for laboratories performing the routine identification of Salmonella strains belonging to commonly encountered serovars and isolated from a given geographical area, assuming that the system has been validated beforehand with a significant number of strains originating from that particular area.

* Corresponding author. Mailing address: Veterinary and Agrochemical Research Centre, Department of Bacteriology and Immunology, Groeselenberg 99, Brussels B-1180, Belgium. Phone: 32 (0)2 3790426. Fax: 32 (0)2 3790670. E-mail: Pierre.Wattiau{at}var.fgov.be

{triangledown} Published ahead of print on 8 October 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, December 2008, p. 4037-4040, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01405-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»