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Journal of Clinical Microbiology, December 2008, p. 4083-4086, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01408-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Simple Disk-Based Method for Detection of Klebsiella pneumoniae Carbapenemase-Type β-Lactamase by Use of a Boronic Acid Compound{triangledown}

Yohei Doi,1* Brian A. Potoski,1,2 Jennifer M. Adams-Haduch,1 Hanna E. Sidjabat,1 Anthony W. Pasculle,1,3 and David L. Paterson1,4

Division of Infectious Diseases,1 Antibiotic Management Program,2 Clinical Microbiology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania,3 University of Queensland, UQ Centre for Clinical Research, and Royal Brisbane and Women's Hospital, Brisbane, Australia4

Received 22 July 2008/ Returned for modification 19 September 2008/ Accepted 8 October 2008

A disk potentiation method using carbapenems as substrates and 3-aminophenyl boronic acid as an inhibitor was evaluated for the detection of Klebsiella pneumoniae carbapenemase (KPC)-type β-lactamases. When combined with nonsusceptibility to ertapenem, the method was easy to perform and reliably differentiated isolates producing KPC-type β-lactamases from those producing other types of β-lactamases.

* Corresponding author. Mailing address: Division of Infectious Diseases, University of Pittsburgh Medical Center, S829 Scaife Hall, 3550 Terrace Street, Pittsburgh, PA 15261. Phone: (412) 648-9445. Fax: (412) 648-8521. E-mail: doiy{at}dom.pitt.edu

{triangledown} Published ahead of print on 15 October 2008.

Journal of Clinical Microbiology, December 2008, p. 4083-4086, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01408-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pasteran, F., Mendez, T., Guerriero, L., Rapoport, M., Corso, A. (2009). Sensitive Screening Tests for Suspected Class A Carbapenemase Production in Species of Enterobacteriaceae. J. Clin. Microbiol. 47: 1631-1639 [Abstract] [Full Text]  


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