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Journal of Clinical Microbiology, December 2008, p. 4087-4090, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01246-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Genotypic Antiretroviral Resistance Testing for Human Immunodeficiency Virus Type 1 Integrase Inhibitors by Use of the TruGene Sequencing System{triangledown} ,{dagger}

Christian Paar,1 Christina Palmetshofer,1 Karin Flieger,2,{ddagger} Maria Geit,3 Rolf Kaiser,4 Herbert Stekel,1 and Jörg Berg1*

Institute of Laboratory Medicine, General Hospital Linz, Krankenhausstrasse 9, A-4020 Linz, Austria,1 Siemens Medical Solutions Diagnostics, Hohe Strasse 4-8, D-61231 Bad Nauheim, Germany,2 Department of Dermatology, General Hospital Linz, Krankenhausstrasse 9, A-4020 Linz, Austria,3 Institute of Virology, University of Cologne, Fürst-Pückler-Strasse, D-5650935 Cologne, Germany4

Received 2 July 2008/ Returned for modification 15 August 2008/ Accepted 3 October 2008

A sequencing assay for detection of mutations conferring resistance to human immunodeficiency virus type 1 (HIV-1) integrase inhibitors raltegravir and elvitegravir was developed using the automated TruGene sequencing system. The assay returned clear sequencing results for samples with ≥500 RNA copies/ml for mutation detection and HIV-1 subtyping across a spectrum of HIV-1 subtypes.

* Corresponding author. Mailing address: Institute of Laboratory Medicine, General Hospital Linz, Krankenhausstrasse 9, A-4020 Linz, Austria. Phone: 43-732-7806-1174. Fax: 43-732-7806-741174. E-mail: joerg.berg{at}akh.linz.at

{triangledown} Published ahead of print on 22 October 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

{ddagger} Present address: Leica Biosystems Nussloch GmbH, Heidelberger Str. 17-19, D-69226 Nussloch, Germany.

Journal of Clinical Microbiology, December 2008, p. 4087-4090, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01246-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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