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Journal of Clinical Microbiology, December 2008, p. 4091-4094, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01349-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Combined Multilocus Short-Sequence-Repeat and Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem-Repeat Typing of Mycobacterium avium subsp. paratuberculosis Isolates{triangledown} ,{ddagger}

Virginie C. Thibault,1 Maggy Grayon,1 Maria Laura Boschiroli,2 Eve Willery,3,6 Caroline Allix-Béguec,4 Karen Stevenson,5 Franck Biet,1,{dagger} and Philip Supply3,6,{dagger}*

INRA, UR1282, Infectiologie Animale, Santé Publique (IASP-311), F-37380 Nouzilly, France,1 Unité Zoonoses Bactériennes, Agence Française de Sécurité Sanitaire des Aliments, 23 av. du Général de Gaulle, F-94706 Maisons-Alfort cedex, France,2 INSERM U629,3 Institut Pasteur de Lille, 1 rue du Professeur Calmette, F-59019 Lille Cedex, France,6 Genoscreen, 1 rue du Professeur Calmette, F-59019 Lille Cedex, France,4 Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland, United Kingdom5

Received 15 July 2008/ Returned for modification 16 August 2008/ Accepted 3 October 2008

Short-sequence-repeat (SSR) sequencing was applied to 127 Mycobacterium avium subsp. paratuberculosis isolates typed by mycobacterial interspersed repetitive unit-variable-number tandem repeats (MIRU-VNTR) and IS900 restriction fragment length polymorphism (RFLP). Combined MIRU-VNTR and SSR typing followed by secondary IS900 RFLP typing is an improved approach to high-resolution genotyping of this pathogen.

* Corresponding author. Mailing address: INSERM U629, Institut Pasteur de Lille, 1 rue du professeur Calmette, 59019 Lille, France. Phone: (33) 3 20871154. Fax: (33) 3 20871158. E-mail: philip.supply{at}ibl.fr

{triangledown} Published ahead of print on 15 October 2008.

{ddagger} Supplemental material for this article may be found at http://jcm.asm.org/.

{dagger} P.S. and F.B. contributed equally to this work.

Journal of Clinical Microbiology, December 2008, p. 4091-4094, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01349-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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