This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Balinsky, C. A.
Right arrow Articles by Rodriguez, L. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Balinsky, C. A.
Right arrow Articles by Rodriguez, L. L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2008, p. 438-442, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01953-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rapid Preclinical Detection of Sheeppox Virus by a Real-Time PCR Assay{triangledown}

C. A. Balinsky,1,3,4 G. Delhon,1,5,6 G. Smoliga,1 M. Prarat,1,2 R. A. French,4 S. J. Geary,3,4 D. L. Rock,1,6 and L. L. Rodriguez1*

Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, New York 11944,1 Plum Island Animal Disease Center, Animal and Plant Health Inspection Service, United States Department of Agriculture, Greenport, New York 11944,2 Center of Excellence for Vaccine Research, University of Connecticut, Storrs, Connecticut 06269,3 Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, Connecticut 06269,4 Area of Virology, School of Veterinary Sciences, University of Buenos Aires, 1427 Buenos Aires, Argentina,5 Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois 618026

Received 3 October 2007/ Returned for modification 21 October 2007/ Accepted 11 November 2007

Sheeppox virus (SPPV) is a member of the Capripoxvirus (CaPV) genus of the Poxviridae family. Members of this genus, which also include goatpox and lumpy skin disease viruses, cause economically significant disease in sheep, goats, and cattle. A rapid diagnostic assay for CaPV would be useful for disease surveillance as well as for detection of CaPV in clinical samples and for outbreak management. Here we describe a fluorogenic probe hydrolysis (TaqMan) PCR assay designed for rapid detection of CaPV and tested on sheep experimentally infected with a virulent strain of SPPV. This assay can detect SPPV in buffy coats, nasal swabs, oral swabs, scabs, and skin lesions as well as in lung and lymph nodes collected at necropsy. This single-tube diagnostic assay can be performed in 2 h or less and can detect viral DNA in preclinical, clinical, and postmortem samples.

* Corresponding author. Mailing address: Plum Island Animal Disease Center, P.O. Box 848, Greenport, NY 11944-0848. Phone: (631) 323-3223. Fax: (631) 323-3006. E-mail: Luis.Rodriguez{at}ars.usda.gov

{triangledown} Published ahead of print on 21 November 2007.

Journal of Clinical Microbiology, February 2008, p. 438-442, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01953-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Balamurugan, V., Jayappa, K. D., Hosamani, M., Bhanuprakash, V., Venkatesan, G., Singh, R. K. (2009). Comparative efficacy of conventional and taqman polymerase chain reaction assays in the detection of capripoxviruses from clinical samples. jvdi 21: 225-231 [Abstract] [Full Text]  
  • Babiuk, S., Bowden, T. R., Parkyn, G., Dalman, B., Hoa, D. M., Long, N. T., Vu, P. P., Bieu, D. X., Copps, J., Boyle, D. B. (2009). Yemen and Vietnam capripoxviruses demonstrate a distinct host preference for goats compared with sheep. J. Gen. Virol. 90: 105-114 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»