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Journal of Clinical Microbiology, February 2008, p. 470-476, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01425-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

eap Gene as Novel Target for Specific Identification of Staphylococcus aureus{triangledown}

Muzaffar Hussain,1 Christof von Eiff,1 Bhanu Sinha,2 Insa Joost,3 Mathias Herrmann,3 Georg Peters,1 and Karsten Becker1*

Institute of Medical Microbiology, University of Münster, 48149 Münster,1 Institute of Hygiene and Microbiology, University of Würzburg, 97080 Würzburg,2 Institute of Medical Microbiology and Hygiene, University of Saarland Hospital, 66421 Homburg/Saar, Germany3

Received 17 July 2007/ Returned for modification 19 October 2007/ Accepted 12 December 2007

The cell surface-associated extracellular adherence protein (Eap) mediates adherence of Staphylococcus aureus to host extracellular matrix components and inhibits inflammation, wound healing, and angiogenesis. A well-characterized collection of S. aureus and non-S. aureus staphylococcal isolates (n = 813) was tested for the presence of the Eap-encoding gene (eap) by PCR to investigate the use of the eap gene as a specific diagnostic tool for identification of S. aureus. Whereas all 597 S. aureus isolates were eap positive, this gene was not detectable in 216 non-S. aureus staphylococcal isolates comprising 47 different species and subspecies of coagulase-negative staphylococci and non-S. aureus coagulase-positive or coagulase-variable staphylococci. Furthermore, non-S. aureus isolates did not express Eap homologs, as verified on the transcriptional and protein levels. Based on these data, the sensitivity and specificity of the newly developed PCR targeting the eap gene were both 100%. Thus, the unique occurrence of Eap in S. aureus offers a promising tool particularly suitable for molecular diagnostics of this pathogen.

* Corresponding author. Mailing address: University of Münster, Institute of Medical Microbiology, D-48149 Münster, Germany. Phone: (49) 251 83-55360. Fax: (49) 251 83-55350. E-mail: kbecker{at}uni-muenster.de

{triangledown} Published ahead of print on 19 December 2007.

Journal of Clinical Microbiology, February 2008, p. 470-476, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01425-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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