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Journal of Clinical Microbiology, February 2008, p. 582-587, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01600-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Multiplex PCR for Rapid Detection of Staphylococcus aureus Isolates Suspected to Represent Community-Acquired Strains{triangledown}

B. Strommenger,* C. Braulke, B. Pasemann, C. Schmidt, and W. Witte

National Reference Centre for Staphylococci, Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany

Received 11 August 2007/ Returned for modification 3 October 2007/ Accepted 9 November 2007

The continuous spread of community-acquired methicillin-resistant Staphylococcus aureus (caMRSA) and the introduction of these highly virulent isolates into hospitals represent increasing threats. The timely recognition of caMRSA strains is crucial for infection control purposes. Thus, we developed a PCR-based assay for the easy and rapid determination of those caMRSA clones that currently are the most prevalent in Germany and Central Europe. This assay was able to correctly identify the majority of the isolates as caMRSA of sequence type 80 (ST80), clonal complex 1 (USA400), and ST8 (USA300). In combination with spa typing-BURP (based upon repeat pattern) analysis and resistance typing, it provides a means for the extensive characterization of suspicious isolates. Thus, this assay represents a reliable tool for monitoring the emergence and spread of different caMRSA clones. The resulting information, in combination with careful interpretation of the epidemiological records, might help to prevent the further spread of those highly virulent caMRSA clones.

* Corresponding author. Mailing address: Robert Koch Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany. Phone: 0049/3943/679 260. Fax: 0049/3943/679 317. E-mail: strommengerb{at}rki.de

{triangledown} Published ahead of print on 21 November 2007.

Journal of Clinical Microbiology, February 2008, p. 582-587, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01600-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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