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Journal of Clinical Microbiology, February 2008, p. 743-746, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.02071-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparison of the BD GeneOhm Methicillin-Resistant Staphylococcus aureus (MRSA) PCR Assay to Culture by Use of BBL CHROMagar MRSA for Detection of MRSA in Nasal Surveillance Cultures from an At-Risk Community Population{triangledown}

Jason E. Farley,1,2* Paul D. Stamper,4 Tracy Ross,4 Mian Cai,4 Sharon Speser,4 and Karen C. Carroll2,3,4

The Johns Hopkins University School of Nursing,1 Division of Infectious Disease,2 Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine,3 Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, Maryland4

Received 24 October 2007/ Accepted 19 November 2007

We compared the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture with BBL CHROMagar MRSA for nasal surveillance among 602 arrestees from the Baltimore City Jail. The sensitivity and specificity were 88.5% and 91.0%, respectively, and after secondary analysis using enrichment broth, they were 89.0% and 91.7%, respectively. Twenty-three of 42 false-positive PCR lysates contained methicillin-susceptible S. aureus.

* Corresponding author. Mailing address: Johns Hopkins University, School of Nursing, 600 N. Wolfe Street, Carnegie 346, Baltimore, MD 21287. Phone: (410) 614-5458. Fax: (410) 614-0888. E-mail: jfarley{at}son.jhmi.edu

{triangledown} Published ahead of print on 5 December 2007.

Journal of Clinical Microbiology, February 2008, p. 743-746, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.02071-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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