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Journal of Clinical Microbiology, February 2008, p. 759-762, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.02009-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Human Parechovirus Type 1, 3, 4, 5, and 6 Detection in Picornavirus Cultures{triangledown}

Michel de Vries,1 Krzysztof Pyrc,1,{dagger} Ron Berkhout,2 Wilma Vermeulen-Oost,2 Ronald Dijkman,1 Maarten F. Jebbink,1 Sylvia Bruisten,2 Ben Berkhout,1 and Lia van der Hoek1*

Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands,1 Public Health Laboratory, Municipal Health Service, Amsterdam, The Netherlands2

Received 12 October 2007/ Returned for modification 26 November 2007/ Accepted 4 December 2007

Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR.

* Corresponding author. Mailing address: Laboratory of Experimental Virology, Academic Medical Center of the University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands. Phone: 31-20-5667510. Fax: 31-20-6916531. E-mail: c.m.vanderhoek{at}amc.uva.nl

{triangledown} Published ahead of print on 12 December 2007.

{dagger} Current address: Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.

Journal of Clinical Microbiology, February 2008, p. 759-762, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.02009-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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