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Journal of Clinical Microbiology, February 2008, p. 776-779, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01720-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Molecular Method for Bartonella Species Identification in Clinical and Environmental Samples{triangledown}

Coral García-Esteban,1,2 Horacio Gil,1* Manuela Rodríguez-Vargas,1 Xeider Gerrikagoitia,3 Jesse Barandika,3 Raquel Escudero,1 Isabel Jado,1 Cristina García-Amil,1 Marta Barral,3 Ana L. García-Pérez,3 Mangesh Bhide,1 and Pedro Anda1

Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain,1 Hospital de Getafe, Getafe, Madrid, Spain,2 Neiker Tecnalia, Vizcaya, Spain3

Received 29 August 2007/ Returned for modification 11 November 2007/ Accepted 9 December 2007

A new, efficient molecular method for detection of Bartonella, based on the 16S-23S rRNA intergenic spacer and 16S rRNA amplification by multiplex PCR combined with reverse line blotting, was designed. This assay could simultaneously detect 20 different known species and other Bartonella species not described previously.

* Corresponding author. Mailing address: Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220-Majadahonda, Madrid, Spain. Phone: 34-918223952. Fax: 34-915097919. E-mail: hgil{at}isciii.es

{triangledown} Published ahead of print on 19 December 2007.

Journal of Clinical Microbiology, February 2008, p. 776-779, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01720-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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