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Journal of Clinical Microbiology, February 2008, p. 785-788, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01114-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rapid and Highly Sensitive Coxsackievirus A Indirect Immunofluorescence Assay Typing Kit for Enterovirus Serotyping{triangledown}

Tsuey-Li Lin, Yi-Syue Li, Chiao-Wei Huang, Chiu-Chu Hsu, Ho-Sheng Wu, Tsan-Chang Tseng, and Chen-Fu Yang*

Center for Research and Diagnostic, Centers for Disease Control, Department of Health, Taiwan, 161 Kunyang Street, Nan-Kang, Taipei, Taiwan

Received 3 June 2007/ Returned for modification 26 July 2007/ Accepted 12 November 2007

We describe the development and evaluation of an indirect immunofluorescence assay (IFA) kit for rapid and sensitive detection of coxsackievirus A2, -4, -5, -6, and -10. This IFA kit was determined to have 95.9 to 100% sensitivity and 95.8 to 97.2% specificity. It also proved to be beneficial in reducing the number of enteroviruses that are untypeable in the clinical virology laboratory.

* Corresponding author. Mailing address: Center for Research and Diagnostic, Centers for Disease Control, Department of Health, Taiwan, 161 Kunyang Street, Nan-Kang, Taipei, Taiwan 115. Phone: (886-2) 2653-1106. Fax: (886-2) 2653-0403. E-mail: cxy1{at}cdc.gov.tw

{triangledown} Published ahead of print on 21 November 2007.

Journal of Clinical Microbiology, February 2008, p. 785-788, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01114-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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