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Evaluation of the One-Step Multiplex Real-Time Reverse Transcription-PCR ProFlu-1 Assay for Detection of Influenza A and Influenza B Viruses and Respiratory Syncytial Viruses in Children

Laboratoire de Virologie, Hôpital Européen Georges Pompidou, and Université Paris Descartes, Paris, France,¹ Laboratoire de Microbiologie, Hôpital Saint Louis, Paris, France,² Urgences Pédiatriques, Hôpital Cochin-Saint Vincent de Paul, Paris, France,³ Laboratoire de Virologie, Hôpital Cochin-Saint Vincent de Paul, and Université Paris Descartes, Paris, France⁴

Received 9 May 2007/ Returned for modification 18 July 2007/ Accepted 20 November 2007

We evaluated the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for the detection of influenza A and influenza B viruses and respiratory syncytial viruses from 353 pediatric nasopharyngeal aspirates. As assessed by comparison with the results of immunofluorescence testing and cell culture, the specificity and the sensitivity of the ProFlu-1 assay ranged from 97% to 100%. In addition, the ProFlu-1 assay amplified 9% of samples not detected by conventional methods.

Published ahead of print on 5 December 2007.