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Journal of Clinical Microbiology, February 2008, p. 792-795, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01470-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Dual-Reporter Phenotypic Assay for Human Immunodeficiency Viruses{triangledown}

Keiko Kajiwara, Eiichi Kodama,* Yasuko Sakagami, Takeshi Naito, and Masao Matsuoka

Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University, 53 Kawaramachi, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan

Received 22 July 2007/ Returned for modification 27 September 2007/ Accepted 5 December 2007

We have established a novel human immunodeficiency virus (HIV) tandem-reporter assay using HIV receptor-transduced NP-2 cells with long terminal repeat-controlled β-galactosidase, inserted internal ribosome entry site, and secretary alkaline phosphatase genes. This assay allows users to detect replication of clinical isolates, indicating its useful application as an HIV phenotypic assay.

* Corresponding author. Mailing address: Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University, 53 Kawaramachi, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. Phone and fax: 81-75-751-3986. E-mail: ekodama{at}virus.kyoto-u.ac.jp

{triangledown} Published ahead of print on 19 December 2007.

Journal of Clinical Microbiology, February 2008, p. 792-795, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01470-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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