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Journal of Clinical Microbiology, February 2008, p. 796-799, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01435-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Colorimetric Detection of Multidrug-Resistant or Extensively Drug-Resistant Tuberculosis by Use of Malachite Green Indicator Dye{triangledown}

Parissa Farnia,1* Mohammad Reza Masjedi,1 Foroozan Mohammadi,1 Payam Tabarsei,1 Poopak Farnia,1 Ali Reza Mohammadzadeh,1 Parvaneh Baghei,1 Mohammad Varahram,1 Sven Hoffner,2 and Ali Akbar Velayati1

Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shaheed Beheshti University of Medical Sciences, Darabad, Tehran 19556, P.O. 19575/154, Iran,1 Department of Bacteriology, Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden2

Received 18 July 2007/ Returned for modification 21 September 2007/ Accepted 9 December 2007

The malachite green microtube (MGMT) susceptibility assay was performed directly on sputum specimens (n = 80) and indirectly on Mycobacterium tuberculosis clinical isolates (n = 60). The technique is based on the malachite green dye, which changes color in response to M. tuberculosis growth. The MGMT assay is simple and rapid and does not require expensive instruments.

* Corresponding author. Mailing address: Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shaheed Bahonar Ave., Darabad, Tehran 19556, P.O. Box 19575/154, Iran. Phone: 98 21 20109505. Fax: 98 21 20109484. E-mail: pfarnia{at}hotmail.com

{triangledown} Published ahead of print on 19 December 2007.

Journal of Clinical Microbiology, February 2008, p. 796-799, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01435-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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