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Journal of Clinical Microbiology, February 2008, p. 800-803, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01787-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Multiplex PCR That Can Distinguish between Immunologically Cross- Reactive Serovar 3, 6, and 8 Actinobacillus pleuropneumoniae Strains{triangledown}

L. Zhou,1 S. C. P. Jones,1 Ø. Angen,2 J. T. Bossé,1 J. H. E. Nash,3 J. Frey,4 R. Zhou,5 H. C. Chen,5 J. S. Kroll,1 A. N. Rycroft,6 and P. R. Langford1*

Department of Paediatrics, Imperial College London, St. Mary's Campus, London W2 1PG, United Kingdom,1 National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, DK-1790 Copenhagen V, Denmark,2 Pathogen Genomics Group, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada,3 Institute of Veterinary Bacteriology, University of Berne, Laenggassstrasse 122, 3012 Berne, Switzerland,4 State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan 430070, People's Republic of China,5 Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, North Mymms, Hertfordshire AL9 7TA, United Kingdom6

Received 7 September 2007/ Returned for modification 5 November 2007/ Accepted 5 December 2007

We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests.

* Corresponding author. Mailing address: Department of Paediatrics, Imperial College London, St. Mary's Campus, London W2 1PG, United Kingdom. Phone: 44-(0)20-7594-3359. Fax: 44-(0)20-7594-3984. E-mail: p.langford{at}imperial.ac.uk

{triangledown} Published ahead of print on 19 December 2007.

Journal of Clinical Microbiology, February 2008, p. 800-803, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01787-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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