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Journal of Clinical Microbiology, March 2008, p. 1005-1008, Vol. 46, No. 3
0095-1137/08/$08.00+0     doi:10.1128/JCM.01573-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Outbreak Caused by a Multidrug-Resistant Klebsiella pneumoniae Clone Carrying bla_VIM-12 in a University Hospital

Department of Clinical Microbiology, Hippokration General Hospital, Thessaloniki,¹ Department of Microbiology, Medical School, University of Thessaly, Larissa,² Department of Microbiology, Medical School, University of Athens, Athens, Greece³

Received 7 August 2007/ Returned for modification 29 August 2007/ Accepted 24 December 2007

From November 2006 to April 2007, nine nonrepetitive isolates of Klebsiella pneumoniae with reduced susceptibility or resistance to carbapenems were recovered from clinical specimens from separate patients hospitalized in a tertiary care hospital. The imipenem-EDTA synergy test was positive for all isolates. PCR, sequencing, and transferability experiments revealed the novel bla_VIM-12 metallo-β-lactamase gene, which was plasmid mediated and located in a class 1 integron. Pulsed-field gel electrophoresis demonstrated a single macrorestriction pattern, indicating the clonal spread of VIM-12-producing K. pneumoniae.

Published ahead of print on 16 January 2008.