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Journal of Clinical Microbiology, March 2008, p. 1098-1100, Vol. 46, No. 3
0095-1137/08/$08.00+0     doi:10.1128/JCM.01926-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Prospective Study of Human Metapneumovirus Detection in Clinical Samples by Use of Light Diagnostics Direct Immunofluorescence Reagent and Real-Time PCR{triangledown}

Marie L. Landry,* Sandra Cohen, and David Ferguson

Department of Laboratory Medicine, Yale New Haven Hospital, and Yale University School of Medicine, New Haven, Connecticut

Received 28 September 2007/ Returned for modification 5 December 2007/ Accepted 20 December 2007

A Light Diagnostics human metapneumovirus (HMPV) monoclonal antibody reagent was evaluated using cytospin-enhanced direct immunofluorescence (DFA), and the results were compared to those for TaqMan reverse transcription-PCR (RT-PCR). Of the 202 samples tested, 48 were positive by RT-PCR, and 41 (85.4%) of these were positive by DFA (P = 0.0771). The commercial availability of an HMPV DFA reagent will be of significant benefit to clinical laboratories.

* Corresponding author. Mailing address: Department of Laboratory Medicine, P.O. Box 208035, Yale University School of Medicine, New Haven, CT 06520-8035. Phone: (203) 688-3475. Fax: (203) 688-8177. E-mail: marie.landry{at}yale.edu

{triangledown} Published ahead of print on 9 January 2008.

Journal of Clinical Microbiology, March 2008, p. 1098-1100, Vol. 46, No. 3
0095-1137/08/$08.00+0     doi:10.1128/JCM.01926-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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