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Journal of Clinical Microbiology, March 2008, p. 1118-1122, Vol. 46, No. 3
0095-1137/08/$08.00+0     doi:10.1128/JCM.01309-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Novel Multiplex PCR Assay for Simultaneous Identification of Community-Associated Methicillin-Resistant Staphylococcus aureus Strains USA300 and USA400 and Detection of mecA and Panton-Valentine Leukocidin Genes, with Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci{triangledown}

Kunyan Zhang,1,2,3,4,5,6* Jo-Ann McClure,1 Sameer Elsayed,2,3,5 Thomas Louie,3,4,5 and John M. Conly1,2,3,4,5,6

Centre for Antimicrobial Resistance, Calgary Health Region, Calgary, Alberta, Calgary Laboratory Services, Calgary, Alberta, and University of Calgary, Calgary, Alberta, Canada,1 Departments of Pathology and Laboratory Medicine,2 Microbiology and Infectious Diseases,3 Medicine,4 Institute of Infection, Immunity and Inflammation, University of Calgary, Calgary, Alberta, Canada,5 Calgary Laboratory Services, Calgary, Alberta, Canada6

Received 29 June 2007/ Returned for modification 26 September 2007/ Accepted 19 December 2007

We developed a novel multiplex PCR assay for rapid identification and discrimination of the USA300 and USA400 strains and concomitant detection of Panton-Valentine leukocidin genes, with simultaneous discrimination of methicillin-resistant Staphylococcus aureus strains from methicillin-susceptible S. aureus strains, S. aureus strains from coagulase-negative staphylococci, and staphylococci from other bacteria.

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, HMRB-B07, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1. Phone: 403-210-8484. Fax: 403-210-7661. E-mail: kzhang{at}ucalgary.ca

{triangledown} Published ahead of print on 26 December 2007.

Journal of Clinical Microbiology, March 2008, p. 1118-1122, Vol. 46, No. 3
0095-1137/08/$08.00+0     doi:10.1128/JCM.01309-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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