This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, K.
Right arrow Articles by Conly, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, K.
Right arrow Articles by Conly, J. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2008, p. 1118-1122, Vol. 46, No. 3
0095-1137/08/$08.00+0     doi:10.1128/JCM.01309-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Novel Multiplex PCR Assay for Simultaneous Identification of Community-Associated Methicillin-Resistant Staphylococcus aureus Strains USA300 and USA400 and Detection of mecA and Panton-Valentine Leukocidin Genes, with Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci{triangledown}

Kunyan Zhang,1,2,3,4,5,6* Jo-Ann McClure,1 Sameer Elsayed,2,3,5 Thomas Louie,3,4,5 and John M. Conly1,2,3,4,5,6

Centre for Antimicrobial Resistance, Calgary Health Region, Calgary, Alberta, Calgary Laboratory Services, Calgary, Alberta, and University of Calgary, Calgary, Alberta, Canada,1 Departments of Pathology and Laboratory Medicine,2 Microbiology and Infectious Diseases,3 Medicine,4 Institute of Infection, Immunity and Inflammation, University of Calgary, Calgary, Alberta, Canada,5 Calgary Laboratory Services, Calgary, Alberta, Canada6

Received 29 June 2007/ Returned for modification 26 September 2007/ Accepted 19 December 2007

We developed a novel multiplex PCR assay for rapid identification and discrimination of the USA300 and USA400 strains and concomitant detection of Panton-Valentine leukocidin genes, with simultaneous discrimination of methicillin-resistant Staphylococcus aureus strains from methicillin-susceptible S. aureus strains, S. aureus strains from coagulase-negative staphylococci, and staphylococci from other bacteria.

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, HMRB-B07, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1. Phone: 403-210-8484. Fax: 403-210-7661. E-mail: kzhang{at}ucalgary.ca

{triangledown} Published ahead of print on 26 December 2007.

Journal of Clinical Microbiology, March 2008, p. 1118-1122, Vol. 46, No. 3
0095-1137/08/$08.00+0     doi:10.1128/JCM.01309-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Larsen, A. R., Goering, R., Stegger, M., Lindsay, J. A., Gould, K. A., Hinds, J., Sorum, M., Westh, H., Boye, K., Skov, R. (2009). Two Distinct Clones of Methicillin-Resistant Staphylococcus aureus (MRSA) with the Same USA300 Pulsed-Field Gel Electrophoresis Profile: a Potential Pitfall for Identification of USA300 Community-Associated MRSA. J. Clin. Microbiol. 47: 3765-3768 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»