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Cytomegalovirus Strain Diversity in Seropositive Women

Department of Pediatrics, The University of Alabama at Birmingham, Birmingham, Alabama,¹ All India Institute of Medical Sciences, New Delhi, India²

Received 26 May 2007/ Returned for modification 10 August 2007/ Accepted 12 January 2008

Infection and reinfection with multiple cytomegalovirus (CMV) strains have been shown to occur in immunocompromised individuals, sexually transmitted disease clinic attendees, and children attending day care centers. To characterize the CMV diversity in healthy seropositive individuals, 16 CMV PCR-positive specimens from 113 seropositive women were analyzed for glycoprotein gN and gB genotypes by cloning, followed by nucleotide sequencing of the plasmid DNA and/or restriction fragment length polymorphism (RFLP). The results showed that most (93.7%) of the PCR-positive specimens contained multiple gN and/or gB genomic variants, suggesting that the majority of women were infected with more than one virus strain. The results also showed that the RFLP technique might not be sufficiently sensitive to detect all of the genomic variants present in a sample.

Published ahead of print on 23 January 2008.