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Journal of Clinical Microbiology, March 2008, p. 882-886, Vol. 46, No. 3
0095-1137/08/$08.00+0     doi:10.1128/JCM.01079-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Cytomegalovirus Strain Diversity in Seropositive Women{triangledown}

Zdenek Novak,1* Shannon A. Ross,1 Raj Kumar Patro,2 Sunil Kumar Pati,2 Rekha A. Kumbla,1 Sallie Brice,1 and Suresh B. Boppana1

Department of Pediatrics, The University of Alabama at Birmingham, Birmingham, Alabama,1 All India Institute of Medical Sciences, New Delhi, India2

Received 26 May 2007/ Returned for modification 10 August 2007/ Accepted 12 January 2008

Infection and reinfection with multiple cytomegalovirus (CMV) strains have been shown to occur in immunocompromised individuals, sexually transmitted disease clinic attendees, and children attending day care centers. To characterize the CMV diversity in healthy seropositive individuals, 16 CMV PCR-positive specimens from 113 seropositive women were analyzed for glycoprotein gN and gB genotypes by cloning, followed by nucleotide sequencing of the plasmid DNA and/or restriction fragment length polymorphism (RFLP). The results showed that most (93.7%) of the PCR-positive specimens contained multiple gN and/or gB genomic variants, suggesting that the majority of women were infected with more than one virus strain. The results also showed that the RFLP technique might not be sufficiently sensitive to detect all of the genomic variants present in a sample.

* Corresponding author. Mailing address: University of Alabama at Birmingham, Children's Hospital, CHB 150, 1600 6th Ave. S., Birmingham, AL 35233. Phone: (205) 996-7896. Fax: (205) 996-7150. E-mail: zdnovak{at}peds.uab.edu

{triangledown} Published ahead of print on 23 January 2008.

Journal of Clinical Microbiology, March 2008, p. 882-886, Vol. 46, No. 3
0095-1137/08/$08.00+0     doi:10.1128/JCM.01079-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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  • Dekker, C. L., Arvin, A. M. (2009). One Step Closer to a CMV Vaccine. NEJM 360: 1250-1252 [Full Text]  
  • Novak, Z., Ross, S. A., Patro, R. K., Pati, S. K., Reddy, M. K., Purser, M., Britt, W. J., Boppana, S. B. (2009). Enzyme-Linked Immunosorbent Assay Method for Detection of Cytomegalovirus Strain-Specific Antibody Responses. CVI 16: 288-290 [Abstract] [Full Text]  
  • Pang, X., Humar, A., Preiksaitis, J. K. (2008). Concurrent Genotyping and Quantitation of Cytomegalovirus gB Genotypes in Solid-Organ-Transplant Recipients by Use of a Real-Time PCR Assay. J. Clin. Microbiol. 46: 4004-4010 [Abstract] [Full Text]  


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